Publications in International Journals / Series (Article (International))
Esterase production by Aureobasidium pullulans URM 7059 in stirred tank and airlift bioreactors using residual biodiesel glycerol as substrate
- Meneses, Dayana P.; Paixão, Livia Maria N.; Fonteles, Thatyane V.; Gudiña, Eduardo J.; Rodrigues, Lígia R.; Fernandes, Fabiano A.N.; Rodrigues, Sueli
- Biochemical Engineering Journal, 168(107954), 2021
Publisher: Elsevier
Abstract
Aureobasidium pullulans URM 7059 produced esterase using residual glycerol from biodiesel as the sole carbon source. The culture medium containing residual glycerol (0.1% v/v), (NH4)2SO4 (4 g/L), and yeast extract (8 g/L) resulted in the highest esterase production using shake-flasks. The enzyme exhibited a molar mass of 50 kDa and was stable at neutral pH and temperatures below 30 °C. The cations Cu2+ and Al3+ did not affect the esterase activity, while Ca2+ promoted the highest activity loss. The enzyme kinetic parameters were determined using different substrates (p-nitrophenylcaprylate and ...
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